Tissue-Specific Expression and Post-Translational Modification of Histone H3 Variants
- 14 August 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 7 (10) , 4225-4236
- https://doi.org/10.1021/pr800044q
Abstract
Analyses of histone H3 from 10 rat tissues using a Middle Down proteomics platform revealed tissue-specific differences in their expression and global PTM abundance. ESI/FTMS with electron capture dissociation showed that, in general, these proteins were hypomodified in heart, liver and testes. H3.3 was hypermodified compared to H3.2 in some, but not all tissues. In addition, a novel rat testes-specific H3 protein was identified with this approach.Keywords
This publication has 65 references indexed in Scilit:
- Lysine Propionylation and Butyrylation Are Novel Post-translational Modifications in HistonesMolecular & Cellular Proteomics, 2007
- Characterization of antimicrobial histone sequences and posttranslational modifications by mass spectrometryJournal of Mass Spectrometry, 2007
- Long-distance combinatorial linkage between methylation and acetylation on histone H3 N terminiProceedings of the National Academy of Sciences, 2007
- Chromatin Modifications and Their FunctionCell, 2007
- N-formylation of lysine in histone proteins as a secondary modification arising from oxidative DNA damageProceedings of the National Academy of Sciences, 2007
- Histone H3 variants and their potential role in indexing mammalian genomes: The “H3 barcode hypothesis”Proceedings of the National Academy of Sciences, 2006
- Polycomb complexes repress developmental regulators in murine embryonic stem cellsNature, 2006
- A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem CellsCell, 2006
- A Blueprint for a Human Epigenome Project: The AACR Human Epigenome WorkshopCancer Research, 2005
- Reading signals on the nucleosome with a new nomenclature for modified histonesNature Structural & Molecular Biology, 2005