Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome
- 1 August 1985
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 92 (4) , 290-296
- https://doi.org/10.1007/bf00329812
Abstract
We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.Keywords
This publication has 55 references indexed in Scilit:
- Dicentric human X chromosomesHereditas, 2009
- Replication and inactivation of and isodicentric X: presence of an inactive centromere influences the replication patternsClinical Genetics, 2008
- The kinetochore is part of the metaphase chromosome scaffold.The Journal of cell biology, 1984
- Isodicentric X chromosome in a moderately tall patient with gonadal dysgenesis: lack of effect of functional centromere on inactivation pattern.Journal of Medical Genetics, 1982
- Centromeric DNA from Saccharomyces cerevisiaeJournal of Molecular Biology, 1982
- Visible light observations on the kinetochore of the Indian muntjac, Muntiacus muntjac, Z.Cytogenetic and Genome Research, 1980
- Replication patterns of three isodicentric X chromosomes and an X isochromosome in human lymphocytesAmerican Journal of Medical Genetics, 1978
- Structure and inheritance of some heterozygous Robertsonian translocation in man.Journal of Medical Genetics, 1976
- New selective Giemsa technique for human chromosomes, Cd stainingNature, 1974
- An abnormal large human chromosome identified as an end‐to‐end fusion of two X's by combined results of the new banding techniques and microdensitometryClinical Genetics, 1972