Diazepam and Desmethyldiazepam Quantitation In Whole Blood By Nitrogen-Sensitive Detector After Derivatization
- 1 September 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 3 (5) , 195-198
- https://doi.org/10.1093/jat/3.5.195
Abstract
A reliable procedure is presented for the quantitation of diazepam and its physiologically active metabolite by use of the nitrogenphosphorous detector (NPD). The problem of quantitation of desmethyldiazepam due to tailing on certain liquid phases such as OV-1 is circumvented by derivatization of the drug to its corresponding “N-propyl” derivative which has a response to NPD detection 1.2 times that of diazapam. Within-run coefficients of variation for drug concentrations of 0.25, 0.50, and 1.5 /µg/mL were between 3 and 8%.Day-to-day variation for the derivatization of desmethyldiazepam at levels of 125 ng/mL and a 750 ng/mL in whole blood was 5% and 7%, respectively.This publication has 2 references indexed in Scilit:
- Monitoring diazepam and desmethyldiazepam concentrations in plasma by gas-liquid chromatography, with use of a nitrogen-sensitive detector.Clinical Chemistry, 1979
- Electron Capture Gas Chromatography Determination of Benzodiazepines and MetabolitesJournal of Analytical Toxicology, 1977