In vitro and in vivo activation of L-serine deaminase in Escherichia coli K-12
- 1 June 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 162 (3) , 1270-1275
- https://doi.org/10.1128/jb.162.3.1270-1275.1985
Abstract
Escherichia coli L-serine deaminase (L-SD) in crude extracts made in glycylglycine could be activated by incubation with iron sulfate and dithiothreitol. This activation could also be demonstrated in vitro in two mutants which were physiologically deficient in L-SD activity in vivo. This suggests that these mutants were deficient not in L-SD but in an enzyme(s) activating L-SD. The suggestion is made that production of a functional L-SD in vivo requires activation of the structural gene product by an enzyme or enzymes that reduce the protein to an active form.This publication has 33 references indexed in Scilit:
- AppppA, heat-shock stress, and cell oxidation.Proceedings of the National Academy of Sciences, 1983
- Interconversion of the molecular forms of biliverdin reductase from rat liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- Sulfate assimilation in Rhodopseudomonas sulfidophilaArchiv für Mikrobiologie, 1983
- Hysteretic activation of methanol-coenzyme M methyltransferase by ATPCanadian Journal of Microbiology, 1982
- In vitro studies on L-serine deaminase activity of Escherichia coli K12Canadian Journal of Biochemistry, 1980
- Reduction of RibonucleotidesAnnual Review of Biochemistry, 1979
- l-serine dehydratase from Arthrobacter globiformisBiochemical Journal, 1977
- Hydrogen donor system for Escherichia coli ribonucleoside-diphosphate reductase dependent upon glutathione.Proceedings of the National Academy of Sciences, 1976
- Glutathione biosynthesis in Escherichia coli K 12 properties of the enzymes and regulationBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- Fenton's reagent revisitedAccounts of Chemical Research, 1975