The nucleotide sequence of spinach chloroplast tryptophan transfer RNA

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Abstract
Spinach chloroplast tRNATrp, purified by column chromatography and two-dimensional gel electrophoresis, has been sequenced using in vitro labeling techniques. The sequence is : pG-C-G-C-U-C-U-U-A-G-U-U-C-A-G-U-U-C-Gm-G-D-A-G-A-A-C-m2G-ψ-C-G-G-ψ-C-U-C-C-A-A*-A-A-C-C-C-G-A-U-G-N-C-G-U-A-G-G-T-ψ-C-A-A-G-U-C-C-U-A-C-A-G-A-G-C-G-U-G-C-C-AOH. Like the E.coli suppressor tRNA psu$UGA which translates both the opal terminator codon U-G-A and the tryptophan codon U-G-G, spinach chloroplast tRNATrp has C-C-A as an anticodon and contains an A-U pair in the D-stem.