GENETICO-BIOCHEMICAL ANALYSIS OF SPECIFIC ESTERASES IN MUSCA DOMESTICA

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Abstract
The various forms of esterases present in tissue extracts of house flies were studied by thin-layer electro-phoresis. These esterases were classified by electrophoretic mobilities, substrate specificity and susceptibility to selective inhibitors. Specific esterases were revealed by using a pH-indicator method which can be applied to any substrate. The esterases hydro-lysing acetylcholine, methyl-n-butyrate, ethyl-n-butyrate, tributyrin, phenylacetate and pnenyl-n-butyrate were compared with esterases splitting [beta] -naphthylacetate, and effects of eserine and TOCP (tri-o-cresyl phosphate) on their zymograms were studied. It was suggested that 4 types of esterases may be distinguished in house fly homogenates. Genetic analysis on a factor responsible for formation of the butylester-splitting enzyme which has low activity in diazinon resistant house flies showed that such activity is controlled by a recessive gene on the 5th chromosome.