Agonists of Toll-Like Receptors 3, 4, 7, and 9 Are Candidates for Use as Adjuvants in an Outer Membrane Vaccine againstNeisseria meningitidisSerogroup B
- 1 December 2007
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 75 (12) , 5939-5946
- https://doi.org/10.1128/iai.00846-07
Abstract
The bacteriumNeisseria meningitidisis the causative agent of meningitis and sepsis. A generally effective vaccine againstN. meningitidisserogroup B is not yet available, but outer membrane vesicle vaccines are in development. These vaccines contain lipopolysaccharide (LPS). The inclusion ofN. meningitidiswild-type LPS in a vaccine is controversial because of its high toxicity. Therefore, the adjuvant activity of a panel of different Toll-like receptor (TLR) agonists in combination with LPS-deficient meningococcal outer membrane complexes was compared after immunization of mice. The results demonstrate that TLR3, TLR4, TLR7, and TLR9 agonists enhance immune responses against LPS-deficient outer membrane complexes. Their adjuvant activity was characterized by higher levels of antigen-specific immunoglobulin G (IgG), IgG2a, and IgG2b; a higher IgG2a/IgG1 ratio; lower total IgE levels; and most importantly, higher serum bactericidal antibody titers compared to LPS-deficient outer membrane complexes alone.Keywords
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