Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos
Open Access
- 11 September 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (17) , 5133-5147
- https://doi.org/10.1093/nar/10.17.5133
Abstract
RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5–10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53±1 bp, 55±1 bp and 270±1 bp upstream from the first coding base and are colinear with the gene. The first pair of molecules represent transcripts initiating at the canonical cap site but the other species represents a transcript over 200 nucleotides longer which initiates upstream of the CAAT and ATAA boxes.Keywords
This publication has 27 references indexed in Scilit:
- The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor.Proceedings of the National Academy of Sciences, 1978
- Characterization and Kinetics of Synthesis of 15S -Globin RNA, a Putative Precursor of -Globin mRNACold Spring Harbor Symposia on Quantitative Biology, 1978
- The rabbit β-globin gene contains a large insert in the coding sequenceCell, 1977
- Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.Proceedings of the National Academy of Sciences, 1977
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Globin RNA precursor molecules: Biosynthesis and processing in erythroid cellsCell, 1977
- Nucleotide sequences at the 5$prime; termini of rabbit $alpha; and $beta; globin mRNACell, 1976
- A precursor of globin messenger RNAJournal of Molecular Biology, 1976
- ENRICHMENT OF ERYTHROCYTES OF FETAL ORIGIN FROM ADULT FETAL BLOOD MIXTURES VIA SELECTIVE HEMOLYSIS OF ADULT BLOOD-CELLS - AID TO ANTENATAL DIAGNOSIS OF HEMOGLOBINOPATHIES1976
- Rapid electrophoresis and quantitation of haemoglobins on cellulose acetateJournal of Clinical Pathology, 1965