Expression of early genes of origin-defective mutants of simian virus 40.
- 1 July 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (7) , 3898-3902
- https://doi.org/10.1073/pnas.77.7.3898
Abstract
The nucleotide sequences of 8 origin-defective mutants of SV40 were determined. All of the mutants suffered deletions, which range in size from 4-241 nucleotides. Some of the mutants induce the synthesis of tumor (T) antigen, others do not. Viral mRNA extracted from rat cells transformed by 2 of the T[tumor]-antigen-positive mutants was analyzed by the S1 nuclease technique of Berk and Sharp. Irrespective of the size or the location of the deletions, the 5'' ends of viral mRNA are located approximately the same distance from the A + T-rich region (A-T-T-T-A-T) rather than at a specific site in the viral genome.Keywords
This publication has 31 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Complete nucleotide sequence of SV40 DNANature, 1978
- The Genome of Simian Virus 40Science, 1978
- Construction of a viable SV40 variant containing two functional origins of DNA replicationCell, 1978
- Spliced early mRNAs of simian virus 40Proceedings of the National Academy of Sciences, 1978
- Virus-specific early RNA in 3T6 cells infected by a tsA mutant of polyoma virusVirology, 1978
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- A novel method to map transcripts: Evidence for homology between an adenovirus mRNA and discrete multiple regions of the viral genomePublished by Elsevier ,1977
- Autoregulation of simian virus 40 gene A by T antigen.Proceedings of the National Academy of Sciences, 1976
- Bidirectional Replication of Simian Virus 40 DNAProceedings of the National Academy of Sciences, 1972