Amino-Acid Sequence of the α and β Chains of Adult Hemoglobin of the Harbor Seal,Phoca vitulina

Abstract
The complete amino-acid sequences of the .alpha. and .beta. chains of adult hemoglobin of harbor seal, Phoca vitulina that belong to carnivora were determined as follows. The .alpha. and .beta. chains isolated by chromatography on a CM-cellulose column were digested with trypsin after S-carboxymethylation. Amino-acid sequences of the tryptic peptides derived from both chains were analysed. Comparing the primary structures of the .alpha. and .beta. chains of the seal hemoglobin with those of human, dog, bear, badger and cat, 19, 12, 12, 11, and 16 substitutions, respectively, were recognized in the .alpha. chain, and 12, 10, 4, 6, and 19 (22) in the .beta. chain.

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