Improvement in HLA‐DQB typing by PCR‐RFLP: Introduction of a constant restriction site in one of the primers for digestion control
- 1 August 1992
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 40 (2) , 86-89
- https://doi.org/10.1111/j.1399-0039.1992.tb01964.x
Abstract
The PCR-RFLP method previously reported by Inoko is a powerful technique for HLA class II typing. The reliability of RFLP interpretation depends on complete digestion by restriction endonucleases using a modified primer with restriction sites as an internal digestion control. The use of restriction enzymes which recognize specific HLA DQB allelic variations makes HLA DQB genotyping possible.Keywords
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