Epstein-Barr Virus and the Somatic Hypermutation of Immunoglobulin Genes in Burkitt's Lymphoma Cells
Open Access
- 1 November 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (21) , 10488-10492
- https://doi.org/10.1128/jvi.75.21.10488-10492.2001
Abstract
It has been suggested that Epstein-Barr virus (EBV) might suppress antibody maturation either by facilitating bypass of the germinal center reaction or by inhibiting hypermutation directly. However, by infecting the Burkitt9s lymphoma (BL) cell line Ramos, which hypermutates constitutively and can be considered a transformed analogue of a germinal center B cell, with EBV as well as by transfecting it with selected EBV latency genes, we demonstrate that expression of EBV gene products does not lead to an inhibition of hypermutation. Moreover, we have identified two natural EBV-positive BL cell lines (ELI-BL and BL16) that hypermutate constitutively. Thus, contrary to expectations, EBV gene products do not appear to affect somatic hypermutation.Keywords
This publication has 45 references indexed in Scilit:
- Cells Expressing the Epstein-Barr Virus Growth Program Are Present in and Restricted to the Naive B-Cell Subset of Healthy TonsilsJournal of Virology, 2000
- Tonsillar memory B cells, latently infected with Epstein–Barr virus, express the restricted pattern of latent genes previously found only in Epstein–Barr virus-associated tumorsProceedings of the National Academy of Sciences, 2000
- The Expression Pattern of Epstein-Barr Virus Latent Genes In Vivo Is Dependent upon the Differentiation Stage of the Infected B CellImmunity, 2000
- EBV-Infected B Cells in Infectious Mononucleosis: Viral Strategies for Spreading in the B Cell Compartment and Establishing LatencyImmunity, 2000
- Mimicry of CD40 Signals by Epstein-Barr Virus LMP1 in B Lymphocyte ResponsesScience, 1999
- Expression of Epstein–Barr Virus Transformation–Associated Genes in Tissues of Patients with EBV Lymphoproliferative DiseaseNew England Journal of Medicine, 1989
- Monoclonal Antibodies to the Latent Membrane Protein of Epstein-Barr Virus Reveal Heterogeneity of the Protein and Inducible Expression in Virus-transformed CellsJournal of General Virology, 1987
- Stable replication of plasmids derived from Epstein–Barr virus in various mammalian cellsNature, 1985
- Paired epstein‐barr virus‐carrying lymphoma and lymphoblastoid cell lines Burkitt's lymphoma patients: Comparative sensitivity to non‐specific and to allo‐specific cytotoxic responses in vitroInternational Journal of Cancer, 1984
- Recovery of Epstein-Barr virus from nonproducer neonatal human lymphoid cell transformantsVirology, 1979