Defective prostaglandin synthesis by C3H/HeJ mouse macrophages stimulated with endotoxin preparations

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Abstract
Macrophages obtained from C3H/HeN mice produced significant amounts of prostaglandin [PG] E when exposed to phenol-extracted [Escherichia coli] lipopolysaccharides (LPS), whereas macrophages from C3H/HeJ mice were unresponsive. The lipid A fraction from phenol-extracted LPS was an effective inducer of PG synthesis by macrophages from C3H/HeN mice. The polysaccharide portion of the LPS molecule had no effect. The C3H/HeJ macrophages did not produce PG E in response to the lipid A moiety of phenol-extracted LPS. LPS prepared by butanol extraction stimulated the production of PG E by macrophages from C3H/HeN and C3H/HeJ mice. The component of butanol-extracted LPS that stimulated the C3H/HeJ macrophages was a lipid A-associated protein. A correlation was found between prostaglandin production by the macrophages of these 2 strains of mice in response to butanol- and phenol-extracted LPS and the lethal effects of the LPS preparations.