A Novel Two-Enzyme Amperometric Electrode for Lactose Determination
- 17 February 2002
- journal article
- Published by Japan Society for Analytical Chemistry in Analytical Sciences
- Vol. 18 (2) , 147-150
- https://doi.org/10.2116/analsci.18.147
Abstract
Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer concentrations as well as the pH on the electrode response were elucidated.Keywords
This publication has 14 references indexed in Scilit:
- Enzymatic membranes for determination of some disaccharides by means of an oxygen electrodeBiosensors and Bioelectronics, 1996
- Purification and Characterization of 6-Phospho-β-galactosidase fromLactobacillus gasseriJCM 1031Bioscience, Biotechnology, and Biochemistry, 1996
- Amperometric enzyme electrode for aerobic glucose monitoring prepared by glucose oxidase immobilized in poly(vinylferrocenium)Journal of Electroanalytical Chemistry, 1995
- Simultaneous determination of lactose and glucose in milk using two working enzyme electrodesTalanta, 1994
- Enzyme electrodes for the determination of carbohydrates in foodSensors and Actuators B: Chemical, 1993
- Modeling the non-steady-state response of an enzyme electrode for lactoseEnzyme and Microbial Technology, 1991
- Development of biosensors for the simultaneous determination of sucrose and glucose, lactose and glucose, and starch and glucoseBiotechnology & Bioengineering, 1991
- Fast responding lactose enzyme electrodeEnzyme and Microbial Technology, 1990
- Flow analysis of lactose and glucose in milk with an improved electrochemical biosensorFood Chemistry, 1990
- Flow-through multi-enzyme electrodes for the determination of lactoseThe Analyst, 1989