SUB-ENDOTHELIAL DEPOSITION OF VON-WILLEBRANDS FACTOR REQUIRES THE PRESENCE OF ENDOTHELIAL-CELLS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 100  (4) , 526-532
Abstract
The presence of FVIII[factor VII]/VWF [von Willebrand factor] in human aortic subendothelium was previously established. The origin of this FVIII/VWF was studied. Indirect immunofluorescence microscopy was employed to detect FVIII/VWF in rabbit aortas, after passage of a balloon catheter. Immediately after this deendothelialization, FVIII/VWF was present directly luminal to the internal elastic lamina. In the subsequent 5 days to 2 mo., no FVIII/VWF was found on the luminal surface of exposed, nonreendothelialized neointima. After endothelial cell regrowth, FVIII/VWF was again seen on the luminal surface. The deposition of subendothelial FVIII/VWF in neointima requires the presence of endothelial cells and that relatively trivial amounts of plasmatic FVIII/VWF are deposited on neointimal connective tissue after deendothelialization.