Electrophoretic identification and quantitation of compounds in the polyphenolic fraction of extra-virgin olive oil
- 16 September 2005
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 26 (18) , 3538-3551
- https://doi.org/10.1002/elps.200500202
Abstract
A capillary zone electrophoresis method has been carried out to determine and quantitate some compounds of the polyphenolic fraction of virgin olive oil which have never previously been determined before using capillary electrophoresis, such as elenolic acid, ligstroside aglycon, oleuropein aglycon, and (+)-pinoresinol. The compounds were identified using standards obtained by semipreparative high-performance liquid chromatography (HPLC). A detailed method optimization was performed to separate the phenolic compounds present in olive oil using a methanol–water extract of Picual extra-virgin olive oil, and different extraction systems were compared (C18-solid phase extraction (SPE), Diol-SPE, Sax-SPE and liquid-liquid extraction). The optimized parameters were 30 mM sodium tetraborate buffer (pH 9.3) at 25 kV with 8 s hydrodynamic injection, and the quantitation was carried out by the use of two reference compounds at two different wavelengths.Keywords
This publication has 29 references indexed in Scilit:
- Qualitative and Semiquantitative Analysis of Phenolic Compounds in Extra Virgin Olive Oils as a Function of the Ripening Degree of Olive Fruits by Different Analytical TechniquesJournal of Agricultural and Food Chemistry, 2004
- Sensitive Determination of Phenolic Acids in Extra-Virgin Olive Oil by Capillary Zone ElectrophoresisJournal of Agricultural and Food Chemistry, 2004
- Comparability and reliability of different techniques for the determination of phenolic compounds in virgin olive oilEuropean Journal of Lipid Science and Technology, 2004
- Main Polyphenols in the Bitter Taste of Virgin Olive Oil. Structural Confirmation by On-Line High-Performance Liquid Chromatography Electrospray Ionization Mass SpectrometryJournal of Agricultural and Food Chemistry, 2003
- Sensory Properties of Virgin Olive Oil Polyphenols: Identification of Deacetoxy-ligstroside Aglycon as a Key Contributor to PungencyJournal of Agricultural and Food Chemistry, 2003
- Use of 1‐acetoxypinoresinol to authenticate Picual olive oilsInternational Journal of Food Science & Technology, 2002
- The development and application of capillary electrophoresis methods for food analysisElectrophoresis, 1999
- Analyses of phenolic compounds by capillary electrophoresis electrospray mass spectrometryRapid Communications in Mass Spectrometry, 1999
- Olive Oil Phenols and Their Potential Effects on Human HealthJournal of Agricultural and Food Chemistry, 1998
- Bitter Taste of Virgin Olive Oil: Correlation of Sensory Evaluation and Instrumental HPLC AnalysisJournal of Food Science, 1989