IGE FORMATION IN RAT FOLLOWING INFECTION WITH NIPPOSTRONGYLUS-BRASILIENSIS .3. SOLUBLE FACTOR FOR GENERATION OF IGE-BEARING LYMPHOCYTES

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 119  (2) , 583-590
Abstract
Normal rat bone marrow cells incubated with serum or lymph from N. brasiliensis(Nb)-infected rats showed an increase in the proportion of Ig[immunoglobulin]E-bearing cells in culture. This effect was produced in a similar fashion by cell-free supernatants (CFS) from cultures of mesenteric lymph node cells obtained from Nb-infected rats. The action of CFS on bone marrow cells appeared specific for the generation of IgE-bearing cells since the proportion of IgM-bearing cells in the culture did not change. The IgE-bearing cells in bone marrow cell cultures consisted of small lymphocytes, blast cells and mast cells, and the addition of CFS to the cultures predominantly increased the number of IgE-bearing blast cells. CFS was effective in increasing the proportion of IgE-bearing small lymphocytes in cultures of normal mesenteric lymph node cells. Removal of IgE in CFS by an anti-IgE immunosorbent did not affect the ability of CFS to generate IgE-bearing cells. The factor(s) in CFS responsible for this activity migrated with serum .beta.-globulins in zone electrophoresis and possessed a molecular size of between 104 and 2 .times. 104 MW. The ability of CFS to generate IgE-bearing cells was diminished by treatment with the enzymes trypsin and RNase A, but was unaffected by chymotrypsin.