TOTAL AND AVAILABLE LYSINE DETERMINATIONS USING HIGH‐PRESSURE LIQUID CHROMATOGRAPHY
- 1 July 1979
- journal article
- research article
- Published by Wiley in Journal of Food Science
- Vol. 44 (4) , 994-997
- https://doi.org/10.1111/j.1365-2621.1979.tb03429.x
Abstract
The use of high pressure liquid chromatography (HPLC) in determining total and available lysine was studied. Protein‐carbohydrate mixtures were analyzed for available lysine by reaction with fluoro‐dinitrobenzene (FDNB) and separation of e‐DNP‐lysine by HPLC. When comparing the HPLC method to an FDNB spectrophotometric method, the latter gave higher results. The difference was attributed to compounds formed during hydrolysis that interfered with the spectrophotometric method. Total lysine was determined in food proteins by reacting hydrolysates with dansyl chloride. HPLC was used to separate and quantify didansyl lysine.This publication has 21 references indexed in Scilit:
- Separation of amino acids by high performance liquid chromatographyAnalytical Chemistry, 1976
- A comparison of chemical methods for the determination of available lysine in various proteinsJournal of Agricultural and Food Chemistry, 1976
- An investigation of hydrolytic techniques for the amino acid analysis of foodstuffsJournal of the Science of Food and Agriculture, 1973
- A collaborative study on the determination of available lysine in feeding stuffsJournal of the Science of Food and Agriculture, 1973
- Problems in the determination of FDNB‐available lysineJournal of the Science of Food and Agriculture, 1971
- Micro-method for determination of non-N-terminal lysine in milk and similar productsJournal of the Science of Food and Agriculture, 1971
- Mechanisms of heat damage in proteinsBritish Journal of Nutrition, 1970
- A rapid chromatographic method for the estimation of lysineJournal of Chromatography A, 1970
- Available lysine. II.—Determination of available lysine in feedingstuffs by dinitrophenylationJournal of the Science of Food and Agriculture, 1968
- Available lysine. I.—Determination of non‐N‐terminal lysine in proteinJournal of the Science of Food and Agriculture, 1968