Citrus Tissue Culture

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Abstract
In vitro growth of explant (juice vesicle or albedo tissues) cultures from citron (C. medica), lemon (C. limon), grapefruit (C. paradisi), sweet orange (C. sinensis) and mandarin (C. reticulata) fruits was stimulated by addition of orange juice (10% vol/vol optimum) to a basal medium containing Murashige and Skoog salts, 50 g/l sucrose, 100 mg/l myo-inositol, 5 mg/l thiamine .cntdot. HCl, 2 mg/l 2,4-D and 0.5 mg/l kinetin. In analyzing this effect of orange juice on citron explant cultures, increased yields were not obtained by addition of appropriate concentrations of citric acid to the basal medium but growth was stimulated when the medium was supplemented with juice from an acidless orange variety (cv. Lima). Apparently some component(s) other than citric acid is involved. Addition of the inorganic ash corresponding to 10% (vol/vol) orange juice to the basal medium had no effect on yields. Similarly, the stimulatory effect of orange juice could not be explained based on its content of sucrose or of organic growth factors already present in the basal medium.