REACTIVITY OF FIBRINOGEN DERIVATIVES WITH ANTISERA TO HUMAN FIBRIN D-DIMER AND ITS GAMMA-GAMMA-CHAIN REMNANT
- 1 January 1982
- journal article
- research article
- Vol. 48 (1) , 33-37
Abstract
Highly purified D-dimer was obtained from plasmin digest of human cross-linked fibrin. After reduction of its disulfide bonds, the .gamma.-.gamma. chain remnant, containing cross-linking site, was then isolated by ion-exchange chromatography on CM-cellulose. Antisera obtained by immunizing rabbits with D-dimer and its .gamma.-.gamma. chain remnant contained a small population of antibodies which specifically reacted with D-dimer. A specific radioimmunoassay system allowing detection and quantitation of D-dimer in the presence of fibrinogen and monomeric fragment D was made possible.This publication has 10 references indexed in Scilit:
- Sites of D-domain interaction in fibrin-derived D dimerBiochemistry, 1980
- DETECTION OF CIRCULATING CROSSLINKED FIBRIN DERIVATIVES BY A HEAT EXTRACTION-SDS GRADIENT GEL-ELECTROPHORETIC TECHNIQUE1979
- ANTIGENIC MARKERS ON FRAGMENT-DD, A UNIQUE PLASMIC DERIVATIVE OF HUMAN CROSSLINKED FIBRIN1979
- The detection of neoantigenic sites on the D-dimer peptide isolated from plasmin digested cross linked fibrinThrombosis Research, 1979
- Modification of high molecular weight plasmic degradation products of human crosslinked fibrinBiochimica et Biophysica Acta (BBA) - Protein Structure, 1978
- Characterization of Serum Fibrinogen and Fibrin Fragments Produced during Disseminated Intravascular CoagulationBritish Journal of Haematology, 1978
- Molecular Aspects of Defibrination in a Case of Amniotic Fluid EmbolismThrombosis and Haemostasis, 1977
- Amino acid sequence studies on artiodactyl fibrinopeptidesArchives of Biochemistry and Biophysics, 1967
- A Method of Trace Iodination of Proteins for Immunologic StudiesInternational Archives of Allergy and Immunology, 1966
- LES PRODUITS DE DEGRADATION DU FIBRINOGENE HUMAIN PAR LA PLASMINE .1. SEPARATION ET PROPRIETES PHYSICO-CHIMIQUES1961