Ro 13-9904: Affinity for penicillin binding proteins and effect on cell wall synthesis.

Abstract
Studies on the binding of Ro 13-9904, a new broad-spectrum cephalosporin, showed that it had higher affinities for PBP [penicillin binding proteins] 1b, 2 and 3 of Escherichia coli (3 > 1b > 2) than cefazolin, cephaloridine, cephalothin or cephalexin. With Haemophilus influenzae, Ro 13-9904 showed highest affinities for PBP 4 and 5 followed by PBP-2. It inhibited total cell wall synthesis at lower concentrations than the other .beta.-lactam antibiotics tested.