Effects of solvent molecular toxicity and microenvironment composition on the Δ1 dehydrogenataon activity of Arthrobacter simplex cells
- 20 January 1991
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 37 (2) , 97-102
- https://doi.org/10.1002/bit.260370202
Abstract
Solvent selection tests were carried out for the Δ1 dehydrogenation of 6-α-methylhydrocortisone-21-acetate by Arthrobacter simplex cells in the presence of organic solvents. Solubility limits were determined for substrate and product in dry and water-saturated solvents and solvent mixtures. Molecular toxicity levels were estimated by measuring the dehydrogenation activity decay of freely suspended cells incubated in solvent-saturated aqueous media. Chloroform and n-decan-1-ol were the best choice of solvent, for both solubility and catalytic stability. High concentrations of water-soluble additives, such as monosodium glutamate, were found to greatly improve the retention of activity in chloroform-saturated media.Keywords
This publication has 15 references indexed in Scilit:
- Stability of the steroid Δ1-dehydrogenation system of Arthrobacter simplex in organic solvent-water two-liquid phase environmentsEnzyme and Microbial Technology, 1988
- Enzymatic oxidoreduction of steroids in two-phase systems: Effects of organic solvents on enzyme kinetics and evaluation of the performance of different reactorsEnzyme and Microbial Technology, 1988
- Effect of interphase mixing on a water–organic solvent two‐liquid phase microbial system: ethanol fermentationJournal of Chemical Technology & Biotechnology, 1988
- Additives and Enzyme StabilityBiocatalysis, 1988
- Permeation of hydrocortisone and hydrocortisone 21-alkyl esters through silicone rubber membranes — relationship to regular solution solubility behaviorJournal of Membrane Science, 1987
- On optimizing organic solvents in multi-liquid-phase biocatalysisTrends in Biotechnology, 1985
- Redox reactions in hydrocortisone transformation by Arthrobacter globiformis cellsThe Journal of Steroid Biochemistry and Molecular Biology, 1985
- Maintenance and operational stability of immobilized Arthrobacter simplex for the ▵1-dehydrogenation of steroidsEnzyme and Microbial Technology, 1985
- Application of photo-crosslinkable resin prepolymers to entrap microbial cells. Effects of increased cell-entrapping gel hydrophobicity on the hydrocortisone ?1-DehydrogenationApplied Microbiology and Biotechnology, 1979
- Structural Approach to Partitioning: Estimation of Steroid Partition Coefficients Based upon Molecular ConstitutionJournal of Pharmaceutical Sciences, 1971