Principles for the construction and operation of a device for rapidly freezing suspensions for cryo‐electron microscopy
- 1 March 1987
- journal article
- research article
- Published by Wiley in Journal of Electron Microscopy Technique
- Vol. 5 (3) , 279-284
- https://doi.org/10.1002/jemt.1060050308
Abstract
Details are given concerning the construction and use of a simple device for preparing samples of frozen‐hydrated biological material embedded in thin films of vitreous ice. The control of humidity around the sample and rate of freezing are of prime importance to obtain good specimens for cryo‐electron microscopy. Symptoms of ill performance of such an apparatus and poor cryo‐manipulation are discussed.Keywords
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