1H‐NMR analysis of the sugar structures of glycoproteins as their pyridylamino derivatives
- 15 December 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 209 (2) , 265-268
- https://doi.org/10.1016/0014-5793(86)81125-5
Abstract
The 1H-NMR spectra of a series of pyridylamino (PA-) derivatives of oligosaccharides were obtained and compared with those of the corresponding asparagine-linked sugar chains in order to elucidate the effect of the PA-group on the chemical shifts of structural-reporter signals. The effects were found to be localized within the two residues from the end group. Thus, the data for asparagine-linked chains in the literature are applicable to PA-derivatives, so the combination of pyridylamination and NMR measurements greatly reduces the time required for structure analysis of sugar chains of glycoproteins, because the isolation and purification of the chains as PA-derivatives are easy and efficient.Keywords
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