A Bicistronic Subgenomic mRNA Encodes both the ORF2 and ORF3 Proteins of Hepatitis E Virus
- 15 June 2006
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 80 (12) , 5919-5926
- https://doi.org/10.1128/jvi.00046-06
Abstract
Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and a single subgenomic RNA. This subgenomic RNA, which was capped, initiated at nucleotide 5122 downstream of the first two methionine codons in ORF3 and was bicistronic; two closely spaced methionine codons in different reading frames were used for the initiation of ORF3 and ORF2 translation.Keywords
This publication has 27 references indexed in Scilit:
- The Open Reading Frame 3 Gene of Hepatitis E Virus Contains a cis -Reactive Element and Encodes a Protein Required for Infection of MacaquesJournal of Virology, 2005
- Identification of Genotype 3 Hepatitis E Virus (HEV) in Serum and Fecal Samples from Pigs in Thailand and Mexico, Where Genotype 1 and 2 HEV Strains Are Prevalent in the Respective Human PopulationsJournal of Clinical Microbiology, 2005
- The ORF3 Protein of Hepatitis E Virus Interacts with Liver-specific α1-Microglobulin and Its Precursor α1-Microglobulin/Bikunin Precursor (AMBP) and Expedites Their Export from the HepatocytePublished by Elsevier ,2004
- In Vitro Replication of Hepatitis E Virus (HEV) Genomes and of an HEV Replicon Expressing Green Fluorescent ProteinJournal of Virology, 2004
- The Phosphorylated Form of the ORF3 Protein of Hepatitis E Virus Interacts with Its Non-glycosylated Form of the Major Capsid Protein, ORF2Journal of Biological Chemistry, 2002
- Virus-Specific mRNA Capping Enzyme Encoded by Hepatitis E VirusJournal of Virology, 2001
- Novel strains of hepatitis E virus identified from humans and other animal species: is hepatitis E a zoonosis?Journal of Hepatology, 2000
- ELISA for Antibody to Hepatitis E Virus (HEV) Based on Complete Open-Reading Frame-2 Protein Expressed in Insect Cells: Identification of HEV Infection in PrimatesThe Journal of Infectious Diseases, 1993
- The scanning model for translation: an update.The Journal of cell biology, 1989
- Evidence for a Virus in Non-A, Non-B Hepatitis Transmitted via the Fecal-Oral RouteIntervirology, 1983