Detection of induced death of embryos in sheep by the rosette inhibition test

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Abstract
The rosette inhibition test was used to monitor the decrease of an early pregnancy factor in 2 groups of pregnant sheep (19-21 days) where embryos were surgically removed or by luteolysis induction with cloprostenol. The rosette inhibition titers of sera taken from sheep of each group declined from high (16-18) to low (8-10) levels within 48 h of treatment. Surgical removal of embryos caused little change in serum progesterone concentration whereas cloprostenol prompted a rapid decrease over the same period. Embryo death can be detected by the rosette inhibition test within 48 h of occurrence, but not necessarily by the measurement of progesterone in blood within this period.
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