Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria
Open Access
- 1 February 1996
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 178 (4) , 1197-1199
- https://doi.org/10.1128/jb.178.4.1197-1199.1996
Abstract
Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycobacteria.Keywords
This publication has 20 references indexed in Scilit:
- rpsL+: a dominant selectable marker for gene replacement in mycobacteriaMolecular Microbiology, 1995
- Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteriaGene, 1993
- Temperature-sensitive mutants of the mycobacterium plasmid pAL5000FEMS Microbiology Letters, 1992
- Temperature-sensitive mutants of the mycobacterium plasmid pAL5000FEMS Microbiology Letters, 1992
- Advances in Mycobacterial Genetics: New Promises for Old DiseasesImmunobiology, 1992
- Structure and functions of the cell envelope in relation to mycobacterial virulence, pathogenicity and multiple drug resistanceResearch in Microbiology, 1991
- Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature‐sensitive pSC101 repliconMolecular Microbiology, 1991
- Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatisMolecular Microbiology, 1990
- Site-specific mutagenesis inLegionella pneumophilaby allelic exchange using counterselectable ColE1 vectorsFEMS Microbiology Letters, 1988
- An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in Gram-negative bacteria by marker exchange-eviction mutagenesisGene, 1987