Application of Continuous Flow Electrophoresis to the Study of the Blood Coagulation Proteins and the Fibrinolytic Enzyme System. I. Normal Human Materials1

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Abstract
Fractionation of normal human plasma by continuous flow electrophoresis, with rigid temperature control, results in 32 fractions containing good yields of the various coagulation proteins in apparently undenatured state. The separation of the clotting factors is by no means complete at the pH employed (barbital buffer, pH 8.6), but the studies suggest that by varying pH and repeating electrophoresis good separation may be obtained in the future. Fibrinogen, profibrinolysin, Hageman Factor, plasma thromboplastin antecedent and "glass" factor appeared in the y area. Proaccelerin appeared in the albumin area. The other factors: prothrombin, proconvertin, plasma thromboplastin component, anti-hemophilic factor, antifibrinolysin, antithrombin, and heparin cofactor spread over the [BETA], a2, and a1, and early albumin areas. Electrophoretic fractionation of certain chemically prepared fractions lead to further purification.