Radioimmunoassay of the Attack Complex of Complement in Serum from Patients with Systemic Lupus Erythematosus
- 20 June 1985
- journal article
- Published by Massachusetts Medical Society in New England Journal of Medicine
- Vol. 312 (25) , 1594-1599
- https://doi.org/10.1056/nejm198506203122502
Abstract
We developed a radioimmunoassay to measure the attack complex of complement (SC5b–9) in serum from patients with systemic lupus erythematosus. The radioimmunoassay used a monoclonal antibody to an antigen of C9, which is absent from native C9 but present on SC5b–9. SC5b–9 was detectable in 13 of 63 normal subjects, with a mean value of 0.5 unit (range, 0 to 4.8), and elevated in 13 of 14 patients with active lupus. Analysis of 108 samples from the patients with lupus revealed a mean value of 10.1 units (range, 0 to 35) when the disease was active and 1.0 unit (range, 0 to 4.3) when it was clinically stable. SC5b–9 was a more sensitive measure of disease activity than C3, C4, or CH50; its specificity was equivalent to that of C3 and C4. SC5b–9 was elevated in serum from 6 of 49 patients with other forms of glomerulonephritis.Keywords
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