Simultaneous determination of nadolol enantiomers in human plasma by high‐performance liquid chromatography using fluorescence‐detection

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Abstract
A high‐performance liquid chromatographic assay is described for the separation and quantification of nadolol isomers in human plasma. The isomers were quantified using reverse‐phase HPLC and fluorometric detection after derivatization with the chiral reagent R(‐)‐1‐(naphthyl)ethylisocyanate [R(‐)‐NEI]. The N‐isopropyl analogue (one isomer) of nadolol was used as the internal standard. The method was reproducible based on precision studies where the percent relative standard deviation was less than 15%. The lower limit of quantitation for each isomer was 2.5 ng/mL. This method was used to evaluate the pharmacokinetic profile of nadolol isomers in human subjects following both single and multiple oral dosing.

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