Imaging of RNA in Bacteria with Self-Ligating Quenched Probes
- 31 July 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 124 (33) , 9686-9687
- https://doi.org/10.1021/ja026649g
Abstract
We report on the application of a new class of oligonucleotide reporter probes, QUAL probes, that “light up” when a nucleophilic phosphorothioate probe binds adjacent to a dabsyl-quenched probe. These self-ligating DNA probes were used for sequence-specific detection of 16S rRNA in Escherichia coli cells. Strong fluorescence was observed only when the phosphorothioate and quenched dabsyl probes bind side-by-side on a 16S rRNA target. The results demonstrate the use of QUAL probes to detect specific RNA sequences in bacterial cells without enzymes and without washing steps.Keywords
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