Ion-Pair Liquid Chromatographic Analysis of Phenylpropanolamine in Plasma and Urine by Post-Column Derivatization with O-Phthalaldehyde
- 1 June 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 8 (8) , 1489-1500
- https://doi.org/10.1080/01483918508067159
Abstract
A high pressure Liquid chromatographic analysis of phenylpropano Lamine in plasma and urine by post-column derivatzaition with o-phthalaldehyde is described. Plasma samples are extracted with methylene chloride under alkaline conditions. Urine is diluted with mobile phase without extraction. Using fluorescence detection, the method is sufficiently sensitive (2 ng/ml in 0.5 ml of plasma and 0.5 mcg/ml in 0.2 ml of urine) so that phenylpropano lamine concentrations in plasma or urine may be measured for up to 24 hours following a 75 mg oral dose. Coefficients of variation for inter-day and intra-day precision are less than 10%.This publication has 7 references indexed in Scilit:
- High-performance liquid chromatographic method for post-column, in-line derivatization with o-phthalaldehyde and fluorometric detection of phenylpropanolamine in human urineJournal of Chromatography A, 1984
- Determination of Phenylpropanolamine in Serum and Urine by High-Performance Liquid ChromatographyJournal of Pharmaceutical Sciences, 1983
- Improved High Pressure Liquid Chromatographic Method for Phenylpropanolamine in Human PlasmaAnalytical Letters, 1983
- High-pressure Liquid Chromatographic Analysis of Phenylpropanolamine in Human Plasma following Derivatization with O-PhthalaldehydeJournal of Pharmaceutical Sciences, 1981
- Simultaneous GLC Determination of Phenylpropanolamine and Chlorpheniramine in Urine Using a Nitrogen Selective DetectorJournal of Pharmaceutical Sciences, 1978
- Electron‐Capture GLC Determination of Phenylpropanolamine as a Pentafluorophenyloxazolidine DerivativeJournal of Pharmaceutical Sciences, 1976