A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.
- 1 March 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (3) , 1371-1375
- https://doi.org/10.1073/pnas.78.3.1371
Abstract
The size and structure of viral RNA species synthesized in nuclei (of human cervical carcinoma HeLa cells) isolated during the early phase of productive infection by adenovirus type 2 were examined by electrophoresis in denaturing polyacrylamide cells and the nuclease S1 assay. The major products of transcription in vitro of early regions 1 and 2 in the adenoviral genome are processed RNA molecules that appear to be correctly spliced in isolated nuclei. Splicing of adenoviral RNA molecules is inhibited when nuclei are preincubated with antibodies from systemic lupus erythematosus patients that immunoprecipitate small nuclear ribonucleoprotein particles. The specificity of these antibodies suggests that ribonucleoprotein particles containing U1 RNA are required for the splicing of the adenoviral RNA sequences which were examined.This publication has 35 references indexed in Scilit:
- Two Novel Classes of Small Ribonucleoproteins Detected by Antibodies Associated with Lupus ErythematosusScience, 1981
- An amazing sequence arrangement at the 5′ ends of adenovirus 2 messenger RNACell, 1977
- Spliced segments at the 5′ terminus of adenovirus 2 late mRNAProceedings of the National Academy of Sciences, 1977
- A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybridsPublished by Elsevier ,1977
- Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamideNucleic Acids Research, 1977
- Adenovirus transcriptionJournal of Molecular Biology, 1976
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- Comparison of viral RNA sequences in adenovirus 2-transformed and lytically infected cellsJournal of Molecular Biology, 1975
- RNA Synthesis and Processing in Adenovirus-infected CellsCold Spring Harbor Symposia on Quantitative Biology, 1974
- Characterization of a Soluble Nuclear Ribonucleoprotein Antigen Reactive with Sle SeraThe Journal of Immunology, 1971