Lactate dehydrogenase isoenzymes in foetal and neonatal tissues

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Abstract
The Existence of isoenzymes, one class of the multiple molecular forms of enzymes, is now well recognized. Isoenzyme distribution patterns, particularly those of lactate dehydrogenase (LDH), have been extensively studied and are often tissue and species specific (Markert & Møller, 1959; Wieland, Pfleiderer, Haupt & Wörner, 1959; Plagemann, Gregory & Wroblewski, 1960; Tsao, 1960). Various techniques, such as zone electrophoresis and column chromatography, have been used to separate isoenzymes of lactate dehydrogenase. Using starch-gel electrophoresis combined with visual demonstration of the isoenzyme patterns, we have detected the usual five zones of activity in human tissues (Latner & Skillen, 1961). They are termed LD1 LD2, LD3, LD4 and LD5 where, according to the terminology we use, the latter is the isoenzyme which migrates furthest towards the anode and is most prominent in heart muscle.