Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp.
Open Access
- 1 April 2000
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 13 (4) , 465-469
- https://doi.org/10.1094/mpmi.2000.13.4.465
Abstract
We have previously shown that tomato Cf-9 induces an Avr9-dependent hypersensitive response (HR) in Nicotiana tabacum and potato. We show here that Cf-4 also induces an Avr4-dependent HR in two tobacco species (N. tabacum and N. benthamiana). The HR induced by Cf-4 and Cf-9 was compared in stable tobacco transgenics by a seedling lethal assay and resistance to recombinant Potato virus X expressing Avr4 or Avr9. We also compared HR induction with Agrobacterium-mediated transient expression. The Cf-4/Avr4 combination induced a more rapid HR than Cf-9/Avr9. Sensitive assays for Cf-9 and Cf-4 function should prove useful for structure/function analyses of these resistance proteins in tobacco.Keywords
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