Use of a Pellet Press to Flatten Chromosome Spreads

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Abstract
Plant cells, unlike animal cells, have cellulose walls the resist flattening. Techniques utilizing hypotonic solutions to treat animal cells produce exceptionally flat, well-spread chromosomes; however, these same techniques are not effective with plant cells. The importance of pressure in flattening chromosomes of plants has long been recognized as a necessary part of any successful technique. Darlington and LaCour (1962) simply state, “the degree of spreading is due to the pressure of the cover slip…” More attention should be given to improving methods of applying pressure on the cover slip.

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