Sequence analysis of a cDNA clone of a gene encoding a component of a putative phosphorylcholine‐specific T suppressor factor and functional property of its gene product

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Abstract
We have isolated a putative phosphorylcholine (PC)‐T cell suppressor factor (TsF) cDNA clone, p6‐5, from a cDNA library of a T hybridoma which constitutively secretes a PC‐TsF in vitro [8]. In the present study, we determined the nucleotide sequence of the p6‐5 gene and found that the p6‐5 sequence is 86% homologous to rat preproelastase 1 gene, one of the serine protease genes. An oligopeptide (14 mer, TsF14) deduced from the p6‐5 sequence was synthesized and antisera against TsF14 were prepared in rabbits. Anti‐TsF14‐conjugated Sepharose 4B specifically absorbed the PC‐TsF activity from the culture supernatant of PC‐TsF‐secreting T hybridomas. In contrast, the binding molecule eluted from the anti‐TsF14‐conjugated Sepharose suppressed the antibody response PC specifically. These results indicated that the p6‐5 polypeptide is a component of the PC‐TsF molecule.