STUDIES ON THE NUTRITION AND PHYSIOLOGY OF PASTEURELLA PESTIS I. A

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Abstract
A chemically defined culture medium which duplicated the amino acid composition of casein was developed for P. pestis. Inocula of 103 to 105 cells per ml. from 10 avirulent and 17 virulent strains gave yields averaging 1 x 109 viable cells per ml. in flask cultures shaken at room temp. (25-30 C) for 48 to 72 hrs. Virulence of the organisms for mice was maintained after 2 to 10 repeated subcultures in this medium. Hematin, blood or incubation under reduced atmospheric pressure was required for surface growth when the medium was solidified with agar.