HPLC Analysis of Oxytetracycline Residues in Honey
- 1 May 1986
- journal article
- research article
- Published by Elsevier in Journal of Food Protection
- Vol. 49 (5) , 383-388
- https://doi.org/10.4315/0362-028x-49.5.383
Abstract
Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.This publication has 3 references indexed in Scilit:
- Determination of 2-Chloroethanol in Honey, Beeswax, and PollenJournal of AOAC INTERNATIONAL, 1983
- Oxytetracycline Residues in Surplus Honey, Brood Nest Honey, and Larvae After Medication of Colonies of Honey Bees, Apis mellifera , with Antibiotic Extender Patties, Sugar Dusts, and Syrup Sprays 13Environmental Entomology, 1981
- Retention mechanisms of tetracyclines on a C8 reversed-phase materialJournal of Chromatography A, 1980