Biochemistry of Nitrate Respiration in Pseudomonas stutzeri I. Aerobic and Nitrate Respiration Routes of Carbohydrate Catabolism

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Abstract
Spangler , W. J. (Oregon State University, Corvallis), and C. M. Gilmour . Biochemistry of nitrate respiration in Pseudomonas stutzeri . I. Aerobic and nitrate respiration routes of carbohydrate catabolism. J. Bacteriol. 91: 245–250. 1966.—The metabolic pathways of glucose catabolism were studied in Pseudomonas stutzeri under aerobic conditions and under conditions of nitrate respiration. Studies on both glucose and gluconate catabolism, by the radiorespirometric method, indicated that these substrates are degraded in the same manner, i.e., the Entner-Doudoroff and pentose phosphate pathways. There appeared to be no major shift in primary metabolic pathways when nitrate was used as the terminal hydrogen acceptor in nitrate respiration as opposed to aerobic respiration with free molecular oxygen. It was shown that glucose is not degraded to any appreciable extent under anaerobic conditions in the absence of nitrate. Tentative evidence suggests that the tricarboxylic acid cycle functions under both conditions of oxygen relationships and that the rate of carbon oxidation via the tricarboxylic acid cycle is slower with nitrate respiration than under aerobic conditions.