Laser-Based Photoacoustic Densitometer for Two-Dimensional Scanning of Thin-Layer Chromatographic Plates
- 1 February 1989
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 43 (2) , 249-253
- https://doi.org/10.1366/0003702894203183
Abstract
Laser-based photoacoustic densitometry was applied to two-dimensional analysis in thin-layer chromatography. An acousto-optic device was used to provide both intensity modulation and a rastering scan. The spatial resolution is 25 × 60 spots for an area of 25 × 50 mm on the thin-layer chromatographic plate. Three detection modes, normal modulation, resonant modulation, and unmodulated rapid scan modes were compared. Detection limits in the most sensitive mode and in the fastest mode are 350 pg with scan time of 153 s and 6.9 ng with scan time of 1.5 s, respectively.Keywords
This publication has 13 references indexed in Scilit:
- Resonant Cell Laser-Based Photoacoustic Densitometer for Thin-Layer ChromatographyApplied Spectroscopy, 1988
- Thin-Layer Chromatography with Flame Ionization DetectionPublished by Springer Nature ,1987
- Light-Scanning Photoacoustic Densitometer Applied to Protein DeterminationAnalytical Sciences, 1986
- Effect of laser beam shape parameters on photothermal deflection densitometersAnalytical Chemistry, 1984
- Photothermal deflection densitometer for thin-layer chromatographyAnalytical Chemistry, 1984
- High-Speed Video Densitometry: Principle and ApplicationsJournal of Liquid Chromatography, 1982
- Quantitative examination of thin-layer chromatography plates by photoacoustic spectroscopyAnalytical Chemistry, 1979
- Photoacoustic cell design: resonant enhancement and background signalsApplied Optics, 1977
- Doppler-shifted reflections of x rays in beam foil spectroscopy: commentsApplied Optics, 1976
- Thin-layer chromatography and photoacoustic spectrometryAnalytical Chemistry, 1975