Cell Damage Associated with Changing the Medium of Mesencephalic Cultures in Serum-Free Medium Is Mediated via N-Methyl-D-Aspartate Receptors
- 1 April 1991
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 56 (4) , 1201-1206
- https://doi.org/10.1111/j.1471-4159.1991.tb11411.x
Abstract
Dopaminergic neurons from embryonic rat mesencephalon were grown in simple serum-free media. The cells develop over a period of several weeks in vitro, particularly between day 14 and day 23. Removing the culture medium and replacing it with fresh medium during this interval caused severe damage to the cultures; this damage is mediated by excitatory amino acids acting through glutamate receptors. Damage could be completely prevented by antagonists of the N-methyl-D-aspartate subtype of glutamate receptor. As expected, medium that contains glutamate (i.e., Ham's F-12 medium) caused damage; however, medium that contains no glutamate or aspartate (i.e., Dulbecco's modified Eagle medium) also caused severe damage, and most of the damage was dependent on the presence of glutamine in the medium. The presence of the antibiotics penicillin and streptomycin greatly enhanced damage caused by medium change.Keywords
This publication has 15 references indexed in Scilit:
- Serum and depolarizing agents cause acute neurotoxicity in cultured cerebellar granule cells: role of the glutamate receptor responsive to N-methyl-D-aspartate.Proceedings of the National Academy of Sciences, 1990
- Induction of delayed synchronized bursts in hippocampal slices by weak sine-wave stimulation; role of the NMDA receptorEpilepsy Research, 1989
- Calcium-mediated neurotoxicity: relationship to specific channel types and role in ischemic damageTrends in Neurosciences, 1988
- MK-801 powerfully protects against N-methyl aspartate neurotoxicityEuropean Journal of Pharmacology, 1987
- Cellular uptake disguises action of L‐glutamate on N‐methyl‐D‐aspartate receptors: With an appendix: Diffusion of transported amino acids into brain slicesBritish Journal of Pharmacology, 1985
- Spinal seizures and excitatory amino acid-mediated synaptic transmissionNeuroscience Letters, 1984
- 2-Amino-5-phosphonovalerate (2APV), a potent and selective antagonist of amino acid-induced and synaptic excitationNeuroscience Letters, 1981
- Reduced inhibition during epileptiform activity in the in vitro hippocampal slice.The Journal of Physiology, 1980
- In vitro maturation of mesencephalic dopaminergic neurons from mouse embryos is enhanced in presence of their striatal target cells.Proceedings of the National Academy of Sciences, 1979
- Growth of a rat neuroblastoma cell line in serum-free supplemented medium.Proceedings of the National Academy of Sciences, 1979