Enzymic Removal and Re-expression of a Histocompatibility Antigen, HL-A 2, at the Surface of Human Peripheral Lymphocytes

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Abstract
The removal by papain of a histocompatibility antigen, HL-A 2, from the surface of intact peripheral blood lymphocytes has been examined. Up to 80% of the antigenic determinant can be removed within 90 minutes. If the cells are reincubated in nutritive medium, re-expression of HL-A 2 is complete within 6 hours. Re-expression is reversibly inhibited by puromycin (50 μg/ml), but actinomycin D (6 μg/ml) only partially inhibited re-expression after 2 hours of prior incubation with the drug.