Single-strand DNA triple-helix formation
- 1 October 1990
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 29 (42) , 9761-9765
- https://doi.org/10.1021/bi00494a001
Abstract
Chemical modification studies provide evidence that single-stranded oligodeoxyribonucleotides can form stable intrastrand triple helices. Two oligonucleotides of opposite polarity were synthesized, each composed of a homopurine-homopyrimidine hairpin stem linked to a pyrimidine sequence which is capable of folding back on the hairpin stem and forming specific Hoosteen hydrogen bonds. Using potassium permanganate as a chemical modification reagent, we have found that two oligodeoxyribonucleotides of sequence composition type 5''-(purine)8(N)4(pyrimidine)8(N)6(pyrimidine)8-3'' and 5''-(pyrimidine)8N6(pyrimidine)8N4(purine)8-3'' undergo dramatic structural changes consistent with intrastrand DNA triple-helix formation induced by lowering the pH or raising the Mg2+ concentration. The intrastrand DNA triple helix is sensitive to base mixmatches.This publication has 14 references indexed in Scilit:
- Magnesium ion-dependent triple-helix structure formed by homopurine-homopyrimidine sequences in supercoiled plasmid DNA.Proceedings of the National Academy of Sciences, 1988
- Sequence-specific binding and photocrosslinking of alpha and beta oligodeoxynucleotides to the major groove of DNA via triple-helix formation.Proceedings of the National Academy of Sciences, 1988
- Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine·guanine base pairsCell, 1987
- Formation of the triple-stranded polynucleotide helix, poly(A.A.U).Proceedings of the National Academy of Sciences, 1987
- Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sitesCell, 1987
- The supercoil-stabilised cruciform of ColE1 is hyper-reactive to osmium tetroxide.The EMBO Journal, 1984
- Determination of Base Sequence in Nucleic Acids with the Electron Microscope. V. The Thymine-Specific Reactions of Osmium Tetroxide with Deoxyribonucleic Acid and Its Components*Biochemistry, 1966
- Selective degradation of thymidine and thymine deoxynucleotidesBiochemical Journal, 1966
- Infrared demonstration of two- and three-strand helix formation between poly C and guanosine mononucleotides and oligonucleotidesBiochemical and Biophysical Research Communications, 1964
- FORMATION OF A THREE-STRANDED POLYNUCLEOTIDE MOLECULEJournal of the American Chemical Society, 1957