A simplified analysis of scatchard plots for systems with two interacting binding sites
- 1 January 1988
- journal article
- research article
- Published by Wiley in Biopolymers
- Vol. 27 (1) , 79-86
- https://doi.org/10.1002/bip.360270106
Abstract
A simple graphical method for calculating stoichiometric and site binding constants for systems with two initially equivalent interacting sites is derived from a modified Scatchard equation. The binding constants can be calculated from Scatchard plots (r/[A] as a function of r) using the values of r/[A] (r is the molar ratio of bound ligands to total protein and [A] is the equilibrium concentration of free ligand) when r = 0 and r = 1 (half‐saturation). The applicability of the method to the adsorption of bilirubin by peptide pendants immobilized on a polyacrylamide support is demonstrated.This publication has 14 references indexed in Scilit:
- Theoretical aspects of DNA-protein interactions: Co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous latticePublished by Elsevier ,2004
- Immobilized replicates of sequence 136–148 of human serum albumin as adsorbents for bilirubinCanadian Journal of Chemistry, 1987
- Cooperative binding to macromolecules. A formal approachAccounts of Chemical Research, 1986
- Ligand-receptor interactions: what we can and cannot learn from binding measurementsTrends in Pharmacological Sciences, 1983
- Numbers of Receptor Sites from Scatchard Graphs: Facts and FantasiesScience, 1982
- Protein affinities for small molecules: Conceptions and misconceptionsArchives of Biochemistry and Biophysics, 1979
- [13] The meaning of scatchard and hill plotsPublished by Elsevier ,1978
- Two techniques for evaluating small molecule-macromolecule binding in complex systemAnalytical Biochemistry, 1975
- Interaction of manganese with fragments, complementary fragment recombinations, and whole molecules of yeast phenylalanine specific transfer RNAJournal of Molecular Biology, 1974
- Properties of graphical representations of multiple classes of binding sitesBiochemistry, 1971