Effect of magnesium ion on the structure of the 5S RNA from Escherichia coli. An imino proton magnetic resonance study of the helix I, IV, and V regions of the molecule
- 18 November 1986
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 25 (23) , 7386-7392
- https://doi.org/10.1021/bi00371a021
Abstract
The imino proton nuclear magnetic resonance spectrum of Escherichia coli 5S ribonuclei acid (RNA) changes when the Mg2+ ion concentration drops below physiological levels. The transition between the physiological and low magnesium spectral forms of 5S RNA has a midpoint at approximately 0.3 mM Mg2+. Many of the most conspicuous changes observed in the downfield spectrum of 5S RNA as the magnesium concentration is reduced are due to adjustments in the structures of helices I and IV and the disappearance of resonances originating in helix V. The binding of ribosomal protein L25 to 5S RNA in the absence of magnesium stabilizes helix V structures.This publication has 10 references indexed in Scilit:
- Gene organization and primary structure of a ribosomal RNA operon from Escherichia coliPublished by Elsevier ,2004
- NMR evidence for dynamic secondary structure in helixes II and III of the 5S RNA of Escherichia coliBiochemistry, 1986
- Assignment of resonances in the downfield proton spectrum of Escherichia coli 5S RNA and its nucleoprotein complexes using components of a ribonuclease-resistant fragmentBiochemistry, 1984
- Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminatorsGene, 1984
- Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.Proceedings of the National Academy of Sciences, 1984
- Open and closed 5 S ribosomal RNA, the only two universal structures encoded in the nucleotide sequencesJournal of Molecular Biology, 1983
- Nuclear Overhauser experiments at 500 MHz on the downfield proton spectrum of a ribonuclease-resistant fragment of 5S ribonucleic acidBiochemistry, 1983
- Nuclear Overhauser experiments at 500 MHz on the downfield proton spectra of 5S ribonucleic acid and its complex with ribosomal protein L25Biochemistry, 1983
- Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleasesBiochemistry, 1981
- A proton-coupled conformational switch of Escherichia coli 5S ribosomal RNA.Proceedings of the National Academy of Sciences, 1980