Studies on the Formation of the Internucleotidic Bond in RNA Synthesis Using Dialkylamino Phosphoroamidites

Abstract
Several N,N-dialkylamlno-cyanoethyl-phosphoroamidites were tested for use in solid-phase synthesis of homo-uridine RNA fragments. N-ethyl-N-me-thylamino protection gave the best results. The corresponding monomer is stable for several months and an average coupling yield of 97 % was obtained after 4 minutes of condensation using this agent.

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