Studies on the Formation of the Internucleotidic Bond in RNA Synthesis Using Dialkylamino Phosphoroamidites
- 1 December 1990
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 9 (8) , 1087-1098
- https://doi.org/10.1080/07328319008046065
Abstract
Several N,N-dialkylamlno-cyanoethyl-phosphoroamidites were tested for use in solid-phase synthesis of homo-uridine RNA fragments. N-ethyl-N-me-thylamino protection gave the best results. The corresponding monomer is stable for several months and an average coupling yield of 97 % was obtained after 4 minutes of condensation using this agent.Keywords
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