Site-specific modification of the lactose operator with acetylaminofluorene
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (15) , 5093-5102
- https://doi.org/10.1093/nar/11.15.5093
Abstract
We have synthesized the tetradecamer GAGCXGATAACAAG containing a part of the sequence of the lactose operator. A guanine base in the sequence is replaced by the adduct of the carcinogen 2-acetylaminofluorene with guanine. Under the standard conditions of de-protection, the fluorene moiety is lost, leaving behind a guanine oxidation product. New conditions of de-protection have been developed which allow the isolation of an oligonucleotide containing the adduct of 2-aminofluorene with guanine. The presence of the amino-fluorene adduct greatly increases retention on reverse phase chromatography and produces a unique pattern of sequencing bands.Keywords
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