Decline in Fertility of Bull Semen with Increase in Storage Time as Influenced by Dilution Rate

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Abstract
Three expts. involving 6607 services were conducted to compare rate of decline in nonreturn rate from day of collection to the next day with semen extended 1:100 and 1:300. The avg. nonreturn rate for semen diluted 1:100 was 68.2% and for semen diluted 1:300, 62.4%. The avg. nonreturn rate with semen used on the day of collection was 68.4% and on the next day was 62.5%. Both these differences are highly significant (P< 0.001). The avg. decline from day of collection to the next day with 1:100 was 3.6% and with 1:300 was 8.2%. The differences in decline with 1:100 and with 1:300 were 4.6, 4.7, and 3.8 percentage units, respectively, for the 3 expts. The interaction of age of semen and dilution level was not significant. These breeding results parallel motility observations reported in the literature.