Pig Intestinal Membrane-Bound Receptor (Guanylyl Cyclase) for Heat-Stable Enterotoxin: cDNA Cloning, Functional Expression, and Characterization
- 1 July 1994
- journal article
- Published by Wiley in Microbiology and Immunology
- Vol. 38 (7) , 535-541
- https://doi.org/10.1111/j.1348-0421.1994.tb01819.x
Abstract
A cDNA encoding the receptor protein for a heat-stable enterotoxin (STa) produced by enterotoxigenic Escherichia coli was cloned from intestinal epithelial cells of a 10-week-old pig. The cDNA had an open reading frame of 3,219 base pairs and coded for a protein with 1,073 amino acid residues. The mature protein consisted of 1,050 amino acid residues with a molecular mass of ca. 121 kDa and was 87% and 82% identical with the human and rat protein, respectively. The CHO cell line overexpressing the pig recombinant STa receptor specifically bound to a photoaffinity-labeled analog of STa and showed marked elevation of the cellular content of cGMP in response to STa.Keywords
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